Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Mechanical overload-induced muscle-derived extracellular vesicles promote adipose tissue lipolysis

doi: 10.1096/fj.202100242R

Figure Lengend Snippet: Human skeletal muscle down-regulate miR-1 associated with release of EV containing miR-1 in response to a hypertrophic stimulus. A, Decreased in miR-1 expression 30 min after a bout of resistance exercise compared to pre-exercise. B, Increased miR-1 abundance from EVs isolated from plasma 30 min post-exercise compared to pre-exercise. C, Representative images of human myotubes with or without electrical pulse stimulation (EPS). Myotubes were stained with a pan myosin antibody to accurately measure myotube diameter. D, Increased human myotube diameter 24 h post-EPS. E, Decreased miR-1 expression in primary human myotubes 24 h post-EPS and in (F) Increased EVs miR-1 abundance from culture media of EPS treated myotubes. G, No change in EV concentration isolated from culture media of myotubes with or without EPS. Data are expressed as mean ± SE (n = 10 for A-B; n = 3 for D-F; n = 7 for G) with significance (P < .05) denoted by an asterisk compared to respective sham

Article Snippet: Human MPCs were differentiated for 5 days with fresh media added prior to electrical pulse stimulation (EPS, Ionoptix C-Pace EP) for 8 h at 12 V, 1 Hz, 2 ms, followed by a 16 h rest period.

Techniques: Expressing, Isolation, Clinical Proteomics, Staining, Concentration Assay